Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A and B ) Protein structure of the glycation site of HbA1c, and normal Hb, respectively. The crystal structure of HbA1c (3B75) and Hb (1LFZ) are from Protein Data Bank. Cyan sphere, water molecule; red sphere, oxygen atom; green sphere, carbon atom; blue sphere, nitrogen atom. In (A), the polar force between glucose and heme and surrounding water is highlighted by blue dashed lines. Regions of interest (ROIs) are highlighted by red dashed circles. ( C and D ) Zoom-in view of the interaction between glucose and porphyrin ring from glycated Hb (C) and normal Hb (D).

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques:

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A and B ) Fluorescence spectra of Hb (0.025 mg/ml) (A) along with HbA1c (0.025 mg/ml) (B), respectively. Excitation wavelength, 447 nm; integration time, 1000 s; band-pass filter, 488 ± 10 nm; power on the sample, 150 μW. 20× air objective. ( C and D ) Time-resolved photoluminescence (PL) measurements of Hb (0.025 mg/ml) (C) and HbA1c (0.025 mg/ml) (D), respectively. a.u., arbitrary units. ( E and F ) Absorption spectra (normalized) of Hb (E) and HbA1c (F), respectively.

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques: Fluorescence

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A and B ) Time-resolved decay curves (normalized) of Hb (A) and HbA1c (B), respectively. int., intensity. ( C ) Merged time-resolved curves (normalized) of Hb and HbA1c. ( D and E ) Time-resolved decay curves (normalized) of oxyHb (D) and oxyHbA1c (E), respectively. ( F ) Merged time-resolved curves (normalized) of oxyHb and oxyHbA1c. ( G ) Proposed excited-state dynamic pathway of Hb when pumped at 520 nm and probed at 780 nm.

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques:

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A ) Time-resolved decay curves (normalized) of standard HbA1c solutions (human whole blood based) at different concentrations. ( B ) Zoom-in view of (A) from delay time of 1 to 4 ps. ( C ) Component s versus different ω from 0 to 2π THz for pure HbA1c and Hb. ( D ) Phasor plot of standard HbA1c solutions of different concentrations when ω = 0.8π THz. ( E ) Calibration curve of standard HbA1c solution at different concentrations (component s versus HbA1c%).

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques:

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A ) Pseudocolor transient absorption images (delay time, 0 ps) of single RBCs with ROIs are highlighted by blue dashed circles. Scale bar, 10 μm. Pump: 520 nm, 2 mW on the sample; probe: 780 nm, 10 mW on the sample. int., intensity. ( B and C ) Time-resolved decay curves (normalized) of ROIs shown in (A). ( D to F ) HbA1c fraction (in single RBCs) distribution along with the fitted glucose concentration from three diabetic whole blood samples. ( G to I ) HbA1c fraction (in single RBCs) distribution along with the derived glucose concentration from three healthy whole blood samples. Curve fitted by .

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques: Concentration Assay, Derivative Assay

Journal: Cost Effectiveness and Resource Allocation

Article Title: The association between diabetes related medical costs and glycemic control: A retrospective analysis

doi: 10.1186/1478-7547-4-1

Figure Lengend Snippet: Identification of individuals included in studyBy HbA 1c levels

Article Snippet: [ ] Outside of the United States, the United Kingdom's National Institute for Clinical Excellence recommends a target HbA 1c goal between 6.5% and 7.5%.

Techniques:

Journal: Cost Effectiveness and Resource Allocation

Article Title: The association between diabetes related medical costs and glycemic control: A retrospective analysis

doi: 10.1186/1478-7547-4-1

Figure Lengend Snippet: Descriptive statistics – By HbA 1c levels

Article Snippet: [ ] Outside of the United States, the United Kingdom's National Institute for Clinical Excellence recommends a target HbA 1c goal between 6.5% and 7.5%.

Techniques: Medications